GENETIC DIVERSITY AND DNA FINGERPRINTING OF DENDROBIUM OFFICINALE BASED ON ISSR AND SCOT MARKERS
Tianyuan Yang, Mengjiao Gao, Saisong Huang, Shun-Han Zhang, X.J. ZHANG, T. LI, Wen‐Guang YU, P. MENG, Qianqian Shi
Abstract
The research and utilization of germplasm resources serves as an important basis for breeding good varieties of the medicinal orchid Dendrobium officinale.In this study, ISSR and SCoT molecular markers were used to study the genetic diversity and genetic relationships of 24 D. officinale germplasms.Ten ISSR primers and 12 SCoT primers amplified 81 and 96 gene loci, of which 81 and 95 loci were polymorphic, respectively.ISSR markers gave a genetic diversity index (H) of 0.3388, Shannon information index (I) of 0.5097, and genetic distance ranging from 0.1749 to 0.9605, with an average of 0.4414; SCoT markers gave an H of 0.3330, I of 0.5043, and genetic distance ranging from 0.1823 to 0.7577, with an average of 0.4331.The results of the UPGMA cluster analysis, PCoA, genetic structure analysis, genetic distance, and geographical distance analysis of the two molecular markers were quite different.Further analysis of ISSR + SCoT showed that the combination of the two markers was complementary, and could better reveal the genetic diversity and genetic relationships of D. officinale.The DNA fingerprints of 24 samples of D. officinale were constructed based on ISSR and SCoT markers.This study provides reliable theoretical support for variety classification, breeding, and germplasm identification of D. officinale.