Detergent-free isolation of CYP450-reductase's FMN-binding domain in <i>E. coli</i> lipid-nanodiscs using a charge-free polymer
Bankala Krishnarjuna, Thirupathi Ravula, Ayyalusamy Ramamoorthy
Abstract
The membrane-anchored flavin mononucleotide binding domain (FBD) of the CYP450 reductase and native lipids were directly extracted from E. coli using charge-free pentyl-inulin polymer.
Topics & Concepts
ChemistryReductaseStereochemistryEnzymeBiochemistryATP Synthase and ATPases ResearchChemical Synthesis and AnalysisComputational Drug Discovery Methods