Arginine vasopressin regulates the renal Na<sup>+</sup>-Cl<sup>–</sup> and Na<sup>+</sup>-K<sup>+</sup>-Cl<sup>–</sup> cotransporters through with-no-lysine kinase 4 and inhibitor 1 phosphorylation
Héctor Carbajal‐Contreras, Adrián Rafael Murillo‐de‐Ozores, Germán Magaña-Ávila, Alejandro Márquez‐Salinas, Laurent Bourqui, Michelle Téllez-Sütterlin, Jessica Paola Bahena-López, Eduardo Cortes-Arroyo, Sebastián González Behn-Eschenburg, Alejandro López‐Saavedra, Norma Vázquez, David H. Ellison, Johannes Loffing, Gerardo Gamba, María Castañeda‐Bueno
Abstract
With-no-lysine kinases regulate the phosphorylation and activity of the Na + -Cl – and Na + -K + -2Cl – cotransporters. This pathway is modulated by arginine vasopressin (AVP). However, the link between AVP and WNK signaling remains unknown. Here, we show that AVP activates WNK4 through increased phosphorylation at putative protein kinase A-regulated sites and decreases its dephosphorylation by protein phosphatase 1. This work increases our understanding of the signaling pathways mediating AVP actions in the kidney.