Protocol for renal ischemia-reperfusion injury by flank incisions in mice
Yung-Ting Cheng, Ya-Chun Tu, Yu‐Hsiang Chou, Chun‐Fu Lai
Abstract
Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier with lesser fluid loss and organ injury. Technical challenges during the dissection of renal pedicles are highlighted. For complete details on the execution of this protocol, please refer to Lai et al. (2014).
Topics & Concepts
MedicineFlankDissection (medical)IschemiaClampAcute kidney injuryKidneyRenal ischemiaRenal injuryKidney diseaseSurgeryReperfusion injuryInternal medicineAnatomyComputer scienceComputer visionClampingAcute Kidney Injury ResearchOrgan Donation and TransplantationOrgan Transplantation Techniques and Outcomes