A reference-grade genome identifies salt-tolerance genes from the salt-secreting mangrove species Avicennia marina
Purushothaman Natarajan, Ashok Kumar Murugesan, Ganesan Govindan, Ayyaru Gopalakrishnan, Kumar Ravichandiran, Purushothaman Duraisamy, Raju Balaji, Tanuja Tanuja, P. Sushree Shyamli, Ajay Parida, Madasamy Parani
Abstract
Water scarcity and salinity are major challenges facing agriculture today, which can be addressed by engineering plants to grow in the boundless seawater. Understanding the mangrove plants at the molecular level will be necessary for developing such highly salt-tolerant agricultural crops. With this objective, we sequenced the genome of a salt-secreting and extraordinarily salt-tolerant mangrove species, Avicennia marina, that grows optimally in 75% seawater and tolerates >250% seawater. Our reference-grade ~457 Mb genome contains 31 scaffolds corresponding to its chromosomes. We identified 31,477 protein-coding genes and a salinome consisting of 3246 salinity-responsive genes and homologs of 614 experimentally validated salinity tolerance genes. The salinome provides a strong foundation to understand the molecular mechanisms of salinity tolerance in plants and breeding crops suitable for seawater farming.