Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications
Robert J. Hearnden, Balraj Sandhar, Vishal Vyas, M. Paula Longhi
Abstract
This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018).
Topics & Concepts
Adipose tissueStromal vascular fractionStromal cellCell biologyDownstream (manufacturing)CellBiologyMesenchymal stem cellFraction (chemistry)Computational biologyChemistryCancer researchBiochemistryEngineeringChromatographyOperations managementAdipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and MetabolismCardiovascular Disease and Adiposity