Caffeine and Theophylline Inhibit β-Galactosidase Activity and Reduce Expression in <i>Escherichia coli</i>
Jesse Horne, Elizabeth Beddingfield, Madison Knapp, Stephanie L. Mitchell, Logan Crawford, Shelby Brooks Mills, Alexandra Wrist, Shuyuan Zhang, Ryan M. Summers
Abstract
. The authors recommend the use of alternative reporter systems when such methylxanthines are expected to be present.
Topics & Concepts
TheophyllineCaffeineEscherichia coliEnzymeBeta-galactosidaselac operonChemistryEnzyme assayBiochemistryBiologyPharmacologyGeneEndocrinologyAmino Acid Enzymes and MetabolismPolyamine Metabolism and ApplicationsEnzyme Structure and Function