Unveiling the enzymatic pathway of UMG-SP2 urethanase: insights into polyurethane degradation at the atomic level
Pedro Paiva, Luís M. C. Teixeira, Ren Wei, Weidong Liu, Gert Weber, Jens Preben Morth, Peter Westh, Allan R. Petersen, Martin B. Johansen, Andreas Sommerfeldt, Alexander Sandahl, Daniel E. Otzen, Pedro Alexandrino Fernandes, Maria J. Ramos
Abstract
). Lastly, we identified point mutations that are expected to enhance the enzyme's turnover for the hydrolysis of urethane bonds by stabilizing the macrodipole of the rate-limiting transition state. These findings expand our current knowledge of urethanases and homolog enzymes from the amidase signature superfamily, paving the way for future research on improving the enzymatic depolymerization of PU plastic materials.
Topics & Concepts
PolyurethaneDegradation (telecommunications)EnzymeMaterials scienceChemistryOrganic chemistryComputer scienceTelecommunicationsPolyamine Metabolism and Applicationsbiodegradable polymer synthesis and propertiesEnzyme-mediated dye degradation