The DNA- and protein-binding properties and cytotoxicity of a new copper(II) hydrazone Schiff base complex
Niladri Biswas, Sandeepta Saha, Barun Kumar Biswas, Manas Chowdhury, Ashikur Rahaman, Vivek Junghare, Swati Mohapatra, Saugata Hazra, Ennio Zangrando, Ruma Roy Choudhury, Chirantan Roy Choudhury
Abstract
A new monomeric copper(II) benzhydrazone complex, [Cu(L)(H2O)(NO3)] (1), has been synthesized using Schiff base derived from quinoline-2-carboxaldehyde and benzhydrazide. Complex 1 was characterized by elemental analysis, FT-IR, UV-Vis spectroscopy along with electrochemical study and single-crystal X-ray crystallography. Single-crystal diffraction study of 1 indicates that the copper(II) ion adopts a distorted square pyramidal (SQP) geometry. The calf-thymus (CT) DNA-binding property of 1 has been evaluated by employing UV-vis and fluorescence spectroscopy, cyclic voltammetry and viscosity studies. The protein-binding ability of 1 was investigated by UV-vis and fluorescence spectroscopy titration using BSA and HSA. The significant interaction of 1 with CT-DNA was further supported by molecular docking approach. Finally, in vitro cytotoxicity of 1 was investigated by MTT assay on the Human Skin Carcinoma cell line A431. All these facts indicate that 1 exhibits a promising anticancer activity.