Identification of Metabolically Quiescent <i>Leishmania mexicana</i> Parasites in Peripheral and Cured Dermal Granulomas Using Stable Isotope Tracing Imaging Mass Spectrometry
Joachim Kloehn, Berin A. Boughton, Eleanor Saunders, Sean O’Callaghan, Katrina J. Binger, Malcolm J. McConville
Abstract
O) labeling and imaging mass spectrometry. Using this approach, we show that lesions contain a patchwork of metabolically distinct parasite populations, while the underlying dermal tissues contain a large population of metabolically quiescent parasites. Quiescent parasites also dominate drug-depleted tissues in healed animals, providing an explanation for failure of some first line drugs to completely eradicate parasites. This approach is broadly applicable to study the metabolic and growth dynamics in other host-pathogen interactions.
Topics & Concepts
Leishmania mexicanaLeishmaniaIn vivoBiologyMicrobiologyDrug resistanceIdentification (biology)Mass spectrometryParasite hostingChemistryEcologyBiotechnologyComputer scienceWorld Wide WebChromatographyResearch on Leishmaniasis StudiesTrypanosoma species research and implicationsInsect and Pesticide Research