PIWI‐Interacting RNA HAAPIR Regulates Cardiomyocyte Death After Myocardial Infarction by Promoting NAT10‐Mediated ac<sup>4</sup>C Acetylation of Tfec mRNA
Kai Wang, Kai Wang, Lu‐Yu Zhou, Fang Liu, Lin Liang, Jie Ju, Peng‐Chao Tian, Cui-Yun Liu, Xin‐Min Li, Xinzhe Chen, Tao Wang, Fei Wang, Shaocong Wang, Jian Zhang, Yu‐Hui Zhang, Jinwei Tian, Kun Wang, Kun Wang
Abstract
Abstract PIWI‐interacting RNAs (piRNAs) are abundantly expressed in heart. However, their functions and molecular mechanisms during myocardial infarction remain unknown. Here, a heart‐apoptosis‐associated piRNA (HAAPIR), which regulates cardiomyocyte apoptosis by targeting N ‐acetyltransferase 10 (NAT10)‐mediated N4‐acetylcytidine (ac 4 C) acetylation of transcription factor EC (Tfec) mRNA transcript, is identified. HAAPIR deletion attenuates ischemia/reperfusion induced myocardial infarction and ameliorate cardiac function compared to WT mice. Mechanistically, HAAPIR directly interacts with NAT10 and enhances ac 4 C acetylation of Tfec mRNA transcript, which increases Tfec expression. TFEC can further upregulate the transcription of BCL2‐interacting killer (Bik), a pro‐apoptotic factor, which results in the accumulation of Bik and progression of cardiomyocyte apoptosis. The findings reveal that piRNA‐mediated ac 4 C acetylation mechanism is involved in the regulation of cardiomyocyte apoptosis. HAAPIR‐NAT10‐TFEC‐BIK signaling axis can be potential target for the reduction of myocardial injury caused by cardiomyocyte apoptosis in ischemia heart diseases.