α-Glucuronosyl and α-glucosyl diacylglycerides, natural killer T cell-activating lipids from bacteria and fungi
Satvika Burugupalli, Catarina F. Almeida, D. Smith, Sayali Shah, Onisha Patel, Jamie Rossjohn, Adam P. Uldrich, Dale I. Godfrey, Spencer J. Williams
Abstract
-tuberculostearic acid) and unsaturated (oleic and vaccenic) acids. The TCR footprints on CD1d loaded with a mycobacterial α-glucuronosyl diacylglyceride were assessed using mutant CD1d molecules and, while similar to that for α-GalCer recognition by a type I NKT TCR, were more sensitive to mutations when α-glucuronosyl diacylglyceride was the antigen. In summary, we provide an efficient approach for synthesis of a broad class of bacterial and fungal α-glycosyl diacylglyceride antigens and demonstrate that they can be recognised by TCRs derived from type I and atypical NKT cells.
Topics & Concepts
CD1DBacteriaReceptorCellBiologyMicrobiologyNatural killer cellNatural killer T cellChemistryBiochemistryT cellImmunologyImmune systemGeneticsCytotoxicityIn vitroImmune Cell Function and InteractionInvertebrate Immune Response MechanismsT-cell and B-cell Immunology