Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in Cells
Tho D. K. Nguyen, Lisa Mellander, Alicia A. Lork, Aurélien Thomen, Mai H. Philipsen, Michael E. Kurczy, Nhu T. N. Phan, Andrew G. Ewing
Abstract
in several vesicles was observed after stimulation. Thus, chemical visualization shows transient opening of vesicles to the exterior of the cell without full release the dopamine cargo. We present a direct calculation for the fraction of neurotransmitter release from combined imaging data. The average vesicular release is 60% of the total catecholamine. An important observation is that extracellular molecules can be introduced to cells during the partial exocytotic release process. This nonendocytic transport process appears to be a general route of entry that might be exploited pharmacologically.
Topics & Concepts
VisualizationNanotechnologyBiophysicsChemistryExocytosisMaterials scienceMembraneComputer scienceBiologyBiochemistryData miningCellular transport and secretionLipid Membrane Structure and BehaviorGlycosylation and Glycoproteins Research