Isolation, expression, and characterization of raw starch degrading α-amylase from a marine lake Bacillus megaterium NL3
Sofi Siti Shofiyah, Dewi Yuliani, Nurul Widya, Fean D. Sarian, Fernita Puspasari, Ocky Karna Radjasa, Ihsanawati Ihsanawati, Dessy Natalia
Abstract
. Despite no additional starch-binding domain, BmaN2 is able to hydrolyze various raw starches, such as wheat, corn, cassava, potato, rice, sago, and canna, in which granular wheat is the preferred substrate for BmaN2. These characteristics indicate that BmaN2 is a promising raw starch degrading enzyme within the subfamily GH13_36.
Topics & Concepts
Bacillus megateriumAmylaseLipaseStarchBiologyHydrolysisSubfamilyFood scienceBacteriaThermophileBiochemistryGlycoside hydrolaseBotanyEnzymeGeneGeneticsEnzyme Production and CharacterizationPhytase and its ApplicationsMicrobial Metabolites in Food Biotechnology