Laser induced breakdown spectroscopy for the rapid detection of SARS-CoV-2 immune response in plasma
Kim Berlo, Weiming Xia, Florentine C. Zwillich, Erin Gibbons, R. Gaudiuso, Ebo Ewusi-Annan, G. R. Chiklis, Noureddine Melikechi
Abstract
As the SARS-CoV-2 pandemic persists, methods that can quickly and reliably confirm infection and immune status is extremely urgently and critically needed. In this contribution we show that combining laser induced breakdown spectroscopy (LIBS) with machine learning can distinguish plasma of donors who previously tested positive for SARS-CoV-2 by RT-PCR from those who did not, with up to 95% accuracy. The samples were also analyzed by LIBS-ICP-MS in tandem mode, implicating a depletion of Zn and Ba in samples of SARS-CoV-2 positive subjects that inversely correlate with CN lines in the LIBS spectra.
Topics & Concepts
Laser-induced breakdown spectroscopySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Immune systemCoronavirus disease 2019 (COVID-19)SpectroscopyPlasma2019-20 coronavirus outbreakVirologyMaterials scienceMedicineChemistryImmunologyInternal medicinePhysicsDiseaseInfectious disease (medical specialty)OutbreakQuantum mechanicsLaser-induced spectroscopy and plasmaMercury impact and mitigation studies