Detection of Plasmid-Mediated Resistance to Metronidazole in Clostridioides difficile from River Water
Alois Čížek, Martina Masaříková, Jan Mareš, Marie Brajerova, Marcela Krůtová
Abstract
The combination of direct plating and culture after enrichment was used in order to gain a spectrum of C. difficile ribotypes present in the water samples. Toxigenic C. difficile ribotypes detected in surface water and in wastewater treatment plants overlapped with those derived from patients with CDI and/or animals. Importantly, a recently discovered plasmid-mediated resistance to metronidazole, a drug used for the treatment of CDI, was detected in C. difficile from river water.
Topics & Concepts
MetronidazoleClostridioidesPlasmidMicrobiologyWastewaterDrug resistanceBiologySurface waterAntibioticsEnvironmental scienceGeneEnvironmental engineeringGeneticsClostridium difficile and Clostridium perfringens researchViral gastroenteritis research and epidemiologyGut microbiota and health