A Two-Level, Intramutant Spectrum Haplotype Profile of Hepatitis C Virus Revealed by Self-Organized Maps
Soledad Delgado, Celia Perales, Carlos García-Crespo, María Eugenia Soria, Isabel Gallego, Ana Isabel de Ávila, Brenda Martínez-González, Lucía Vázquez-Sirvent, Cecilio López-Galíndez, Federico Morán, Esteban Domingo
Abstract
The study provides for the first time the haplotype profile and its variation in the course of virus adaptation to a cell culture environment in the absence of external selective constraints. The deep sequencing-based self-organized maps document a two-layer haplotype distribution with an ample basal platform and a lower number of protruding peaks. The results suggest an inferred intramutant spectrum fitness landscape structure that offers potential benefits for virus resilience to mutational inputs.
Topics & Concepts
HaplotypeBiologyGeneticsHepatitis C virusGenomeMutantCoding regionPopulationVirusNucleotide diversityVirologyMutation rateMutationHepacivirus1000 Genomes ProjectHepatitis B virusViral quasispeciesGeneGenetic variationDeep sequencingAmpliconGenotypeFlaviviridaeViral replicationSequence analysisSingle-nucleotide polymorphismPopulation geneticsNucleic acid sequenceHepatitis C virus researchAdvanced Biosensing Techniques and ApplicationsGene expression and cancer classification