Review: tomographic imaging flow cytometry
Andreas Kleiber, Daniel Kraus, Thomas Henkel, Wolfgang Fritzsche
Abstract
Within the last decades, conventional flow cytometry (FC) has evolved as a powerful measurement method in clinical diagnostics, biology, life sciences and healthcare. Imaging flow cytometry (IFC) extends the power of traditional FC by adding high resolution optical and spectroscopic information. However, the conventional IFC only provides a 2D projection of a 3D object. To overcome this limitation, tomographic imaging flow cytometry (tIFC) was developed to access 3D information about the target particles. The goal of tIFC is to visualize surfaces and internal structures in a holistic way. This review article gives an overview of the past and current developments in tIFC.
Topics & Concepts
Tomographic reconstructionFlow cytometryTomographyBiomedical engineeringMedicineRadiologyImmunologyMicrofluidic and Bio-sensing TechnologiesCell Image Analysis TechniquesElectrical and Bioimpedance Tomography