Creation, effects on embryo quality, and clinical outcomes of a new embryo culture medium with 31 optimized components derived from human oviduct fluid: A prospective multicenter randomized trial
Takafumi Utsunomiya, Tatsuma Yao, Hiroko Itoh, Yufuko Kai, Yoko Kumasako, Miwa Setoguchi, Naomi Nakagata, Hiroyuki Abé, Motoharu Ishikawa, Koichi Kyono, Hiroaki Shibahara, Osamu Tsutsumi, Yukihiro Terada, Shunsaku Fujii, Kaoru Yanagida, Minesuke Yokoyama, Sueo NIIMURA, Tsuyoshi Endo, Yoshinori Fukuda, Masato Inoue, Tomohiro Kono, Naoaki Kuji, Fumiko Tawara, Hiroaki Yoshida, Yoshimasa Yokota, Yoshihiro Tada
Abstract
Purpose: Our aim is to make an ideal embryo culture medium close to human oviduct fluid (HOF) components, and to evaluate the quality of this medium with embryo quality and clinical outcomes in assisted reproductive technology (ART) by a prospective randomized controlled trial (RCT). Methods: " (OVIT) was designed. Study II: Embryos (2 pronuclei (2PN) = 9633) were assigned from 1435 patients. The blastulation rate (BR), good BR (gBR), utilized (transferred/cryo-preserved) BR (uBR), pregnancy rate (PR), and miscarriage rate (MR) were compared between the OVIT and control groups by RCT. Results: The novel medium 'OVIT' was produced according to 31 HOF components. The concentrations of essential amino acids (e-AAs) were lower in OVIT than in current media, yet the opposite was true for ne-AA concentrations. gBR and uBR were higher in the OVIT group than in the control group. In the older female group, gBT and uBR were significantly higher in the OVIT group. Conclusions: The novel medium 'OVIT' was produced according to HOF data. The OVIT had significantly better embryo quality and clinical outcomes than the current media.