Laccase-Mediated Flavonoid Polymerization Leads to the Pericarp Browning of Litchi Fruit
Junbin Wei, Xin Zhang, Ruihao Zhong, Bin Liu, Xuelian Zhang, Fang Fang, Zhaoqi Zhang, Xuequn Pang
Abstract
Litchi pericarp turns brown rapidly after fruit harvest, while the mechanism remains obscure. The contents of (−)-epicatechin (EC) and procyanidins (PCs) A2/B1/B2/C1 decreased during the pericarp browning, and a previously identified laccase (ADE/LAC) showed activity to these compounds, with brown products observed in the reactions. By UPLC–DAD-QTOF-MS/MS, isomers of dimeric, trimeric, and tetrameric PCs were detected in the EC-ADE/LAC reaction. In the presence of cyanidin-3-O-rutiside and rutin, anthocyanin-EC and rutin-EC adducts were, respectively, produced, and darker brown precipitation was observed in these reactions relatively to the EC-ADE/LAC reaction alone. ADE/LAC catalyzed the conversion of PC B2 to A-type PC dimers and B-type PC tetramers. ADE/LAC complemented the transparent testa of Arabidopsis LAC15-loss-of-function mutant (tt10) to wild-type dark brown seed coat. The results demonstrated that ADE/LAC-mediated flavonoid polymerization played an important role in the browning of pericarp.