Synthesis and characterization of cerium oxide-bacteriocin nanohybride with synergistic biological activities
Sana M.H. Al-Shimmary, Zina Hashem Shehab, Emad Hamdi Jassim
Abstract
The creation of adjuvants that can increase the effectiveness of therapeutically accessible antibiotics is the overall strategy due to the limited therapeutic choices available for treating multidrug-resistant (MDR) infections. Thus, the goal of this work was to create a novel cerium oxide-bacteriocin nanohybrid (CeO2-bacteriocin nanohybrid), which was purified from Lactobacillus acidophilus (L. acidophilus) and may help bacteriocins become more bioactive and overcome several obstacles. Characterization of nanohybrids via several methods has been performed. X-ray diffraction (XRD) revealed an average crystallite size of 37.19 nm. Transmission electron microscopy (TEM) confirmed that the CeO2-bacteriocin nanohybrid was composed of regular spheres and oval shapes with sizes ranging from 6 to 61 nm. Scanning electron microscopy (SEM) demonstrated that the structure of the CeO2-bacteriocin nanohybrid tended to be spherical, with sizes varying between 45 and 65 nm. As a result, the CeO2-bacteriocin nanohybrid showed strong antibacterial activity than bacteriocin alone, there were highly significant (p ≤ 0.01)differences in the effects of the bacteriocin alone and nanohybrid on the growth of each tested bacteria Minimum Inhibition Concentration and Minimum Bactericidal Concentration against MDR Staphylococcus aureus and XDR Acinetobacter baumannii were recorded. The CeO2-bacteriocin nanohybrid showed promising antibiofilm activity against all the tested bacteria. The new nanohybrid is considered a promising choice as an antioxidant compound for DPPH (2,2-diphenyl-1-picrylhydrazyl) and in vitro cytotoxicity against breast cancer cells (MDA-MB-231 cells).