Tomato Yellow Leaf Curl Virus V2 Protein Plays a Critical Role in the Nuclear Export of V1 Protein and Viral Systemic Infection
Wenhao Zhao, Shuhua Wu, Elizabeth Barton, Yongjian Fan, Yinghua Ji, Xiaofeng Wang, Yijun Zhou
Abstract
mutation is incorporated into a TYLCV infectious clone, the TYLCV-C85S causes delayed onset of very mild symptoms compared to wild-type TYLCV, suggesting that the V1-V2 interaction and, thus, the V2-mediated nuclear export of the V1 protein is crucial for viral spread and systemic infection. Our data point to a critical role of the V2 protein in promoting the nuclear export of the V1 protein and viral systemic infection, likely by promoting V1 protein-mediated nucleocytoplasmic transportation of TYLCV genomic DNA.
Topics & Concepts
BiologyNuclear localization sequenceNuclear transportNuclear export signalViral proteinVirologyCytoplasmTomato yellow leaf curl virusVirusFusion proteinNuclear proteinCell biologyGeminiviridaeNucleusViral replicationViral structural proteinGeneCell nucleusViral entryPlant virusGeneticsBegomovirusTranscription factorRecombinant DNAPlant Virus Research StudiesAnimal Virus Infections StudiesInsect-Plant Interactions and Control