Simple, functional, inexpensive cell extract for in vitro prototyping of proteins with disulfide bonds
Jared L. Dopp, Nigel F. Reuel
Topics & Concepts
Protein disulfide-isomeraseBiochemistryProtein engineeringIsomeraseEnzymeChemistryLuciferasePeriplasmic spaceDirected evolutionDisulfide bondIn vitroLuciferinCell-free protein synthesisCombinatorial chemistryEscherichia coliProtein biosynthesisMutantGeneTransfectionViral Infectious Diseases and Gene Expression in InsectsCRISPR and Genetic EngineeringVirus-based gene therapy research