Quercetin Alleviates Pyroptosis and Necroptosis Triggered on by DEHP Exposure in Bursa of Fabricius in Chicken by the ROS/MAPK/NF-κB Pathway
Chenxi Huang, Xiaodan Wang, Wenwen Zhang, Meichen Liu, Ruirui Xie, Huiling Zheng, Xinyu Huo, Tong Xu, Hongjin Lin
Abstract
Bis(2-ethylhexyl) phthalate (DEHP) is an endocrine disruptor that may cause damage to several species. Quercetin (Que), a common flavonoid, has anti-inflammation, antioxidation, and immune regulation properties. In this study, we identified DEHP-exposed or Que-antagonist groups in chicken and MSB-1 cells to explore whether Que can mitigate DEHP-caused bursa of Fabricius pyroptosis and necroptosis. The findings demonstrated that Que reduced the expression of necroptosis and pyroptosis, inhibited the mitogen-activated protein kinase (MAPK)/NF-κB pathway, and mitigated oxidative stress caused by DEHP. The addition of the reactive oxygen species activator (Sanguinarin) raised the extent of oxidative stress, and the NF-κB activator (nuclear factor-kappa-B activator1, NF-κB act1) activated the MAPK/NF-κB pathway compared to the Que + DEHP group. In conclusion, Que inhibited the MAPK/NF-κB pathway to counteract DEHP-induced bursa pyroptosis and programmed necrosis. This work adds to the toxicological consequences of DEHP on avian further theoretical justification for Que's treatment of organic toxic damage.