Probing the 14-3-3 Isoform-Specificity Profile of Protein–Protein Interactions Stabilized by Fusicoccin A
Ananya Sengupta, Josue Liriano, Ewa A. Bienkiewicz, Brian G. Miller, James H. Frederich
Abstract
. Our results reveal modest isoform preferences for individual client phospholigands and demonstrate that FC differentially stabilizes PPIs involving 14-3-3σ. Together, these data support the feasibility of developing FC variants with enhanced isoform selectivity.
Topics & Concepts
Gene isoformPhosphoproteinFusicoccinSignal transducing adaptor proteinIn vivo14-3-3 proteinBiologyBiochemistryRecombinant DNACell biologyIn vitroComputational biologyReceptorChemistryPhosphorylationGeneGeneticsEnzymeATPase14-3-3 protein interactionsMicrobial Natural Products and BiosynthesisUbiquitin and proteasome pathways