Rapid determination and optimisation of berberine from Himalayan <i>Berberis lycium</i> by soxhlet apparatus using CCD-RSM and its quality control as a potential candidate for COVID-19
Anil Kumar Katare, Bikarma Singh, Bikarma Singh, Pooja Shukla, Sandeep Gupta, Bishander Singh, Bishander Singh, Kavya Yalamanchili, Nitin Kulshrestha, Rajendra Bhanwaria, Ashok Sharma, Sarita Sharma, Sneha, Durga Prasad Mindala, Sumit Roy, Rahul Kalgotra
Abstract
Royle growing in subtropical regions of Asia had wide applications in Indian system of medicine. Rapid determination and novel optimisation method for berberine extraction has been developed by Soxhlet extraction utilising central composite design-response surface methodology (CCD-RSM). Berberine was detected by high-performance liquid chromatography (HPLC), and the highest yield (13.39%) was obtained by maintaining optimal extraction conditions i.e., extraction time (7.28 hrs), ethyl alcohol (52.21%) and solvent to sample ratio (21.78 v/w). Investigation of two geographic regions (Ramnagar and Srinagar) showed high berberine content in lower altitude. This novel optimisation technique has placed berberine as a potential candidate for developing pharmaceutical products for human health care.