Immunosuppressive Functions of M2 Macrophages Derived from iPSCs of Patients with ALS and Healthy Controls
Weihua Zhao, David R. Beers, Jason R. Thonhoff, Aaron D. Thome, Alireza Faridar, Jinghong Wang, Shixiang Wen, Loren Ornelas, Dhruv Sareen, Helen S. Goodridge, Clive N. Svendsen, Stanley H. Appel
Abstract
regulatory T cells (Tregs) and rescued Tregs of ALS patients from losing CD25 and Foxp3. Furthermore, Tregs induced or rescued by iPSC-derived M2 had strong suppressive functions. ALS iPSC-derived M2 cells including those with C9orf72 mutation had similar immunomodulatory activity as control iPSC-derived M2 cells. This study demonstrates that M2 cells differentiated from iPSCs of ALS patients are immunosuppressive, boost ALS Tregs, and may serve as a candidate for immune-cell-based therapy to mitigate inflammation in ALS.
Topics & Concepts
Induced pluripotent stem cellFOXP3Amyotrophic lateral sclerosisIL-2 receptorInflammationImmune systemImmunologyRegulatory T cellC9orf72Cancer researchT cellMedicineBiologyCell biologyDiseaseInternal medicineGeneticsGeneEmbryonic stem cellDementiaFrontotemporal dementiaAmyotrophic Lateral Sclerosis ResearchNeuroinflammation and Neurodegeneration MechanismsImmune cells in cancer