Sensitivity of the amide I band to matrix manipulation in bone: a Raman micro-spectroscopy and spatially offset Raman spectroscopy study
Rafay Ahmed, Mustafa Ünal, Rekha Gautam, Sasidhar Uppuganti, Shrey Derasari, Anita Mahadevan‐Jansen, Jeffry S. Nyman
Abstract
when spectra were acquired through layers of the tissue mimic with a thickness ≤2 mm by the 7 mm offset ring, but not with the 5 mm or 6 mm offset ring. Overall, the SORS instrument was more sensitive than the conventional μRS instrument to pressure- and temperature-related changes in the organic matrix that affect the fracture resistance of bone, but SORS analysis of the amide I band is limited to an overlying thickness layer of 2 mm.
Topics & Concepts
Raman spectroscopyChemistrySpectroscopyAmideRepeatabilityAnalytical Chemistry (journal)OpticsChromatographyPhysicsQuantum mechanicsOrganic chemistrySpectroscopy Techniques in Biomedical and Chemical ResearchDental Implant Techniques and OutcomesMolecular Biology Techniques and Applications