Machine learning-guided engineering of chalcone synthase enables high-selectivity phloretin biosynthesis in yeast
Minyong Li, Canyu Zhang, Hui Liang, Boyu Wu, Wenxi Yu, Guangjian Li, Yufei Cao, Wen‐Yong Lou
Abstract
Machine learning-guided engineering of chalcone synthase effectively overcomes substrate promiscuity, enabling highly selective biosynthesis of phloretin in yeast, and provides a framework for the microbial production of high-value natural products.
Topics & Concepts
Chalcone synthaseBiosynthesisYeastChalconeChemistrySelectivityPhloretinATP synthaseMetabolic engineeringBiochemistryCombinatorial chemistryOrganic chemistryEnzymeCatalysisPlant Gene Expression AnalysisPlant biochemistry and biosynthesisPlant-Microbe Interactions and Immunity