Cinnamic acid regulates the intestinal microbiome and short-chain fatty acids to treat slow transit constipation
Jinguang Jiang, Qian Luo, Shuangshuang Li, Tian-Ying Tan, Kai Xiong, Tao Yang, Tianbao Xiao
Abstract
BACKGROUND: (Xuan Shen), with low toxicity and biological activities to modulate the intestinal microbiome. AIM: To explore the potential effects of CA on the intestinal microbiome and the primary endogenous metabolites-short-chain fatty acids (SCFAs) and evaluate the therapeutic effects of CA in STC. METHODS: Loperamide was applied to induce STC in mice. The treatment effects of CA on STC mice were assessed from the 24 h defecations, fecal moisture and intestinal transit rate. The enteric neurotransmitters: 5-hydroxytryptamine (5-HT) and vasoactive intestinal peptide (VIP) were determined by the enzyme-linked immunosorbent assay. Hematoxylin-eosin and Alcian blue and Periodic acid Schiff staining were used to evaluate intestinal mucosa's histopathological performance and secretory function. 16S rDNA was employed to analyze the composition and abundance of the intestinal microbiome. The SCFAs in stool samples were quantitatively detected by gas chromatography-mass spectrometry. RESULTS: were involved in the production of AA, BA, PA and VA. CONCLUSION: CA could treat STC effectively by ameliorating the composition and abundance of the intestinal microbiome to regulate the production of SCFAs.