Molecular identification of some <i>Fusarium</i> isolates and their chemotypes involved in fusarium head blight on Durum wheat in Algeria
Salah Hadjout, Sylvain Chéreau, Leila Mekliche, Gisel Marchegay, Christine Ducos, Houda Boureghda, Mohamed Zouidi, Christian Barreau, Zouaoui Bouznad, Florence Richard‐Forget
Abstract
The present study reports the species identification of Fusarium isolates infecting Durum wheat cultivated in Algeria. Characterization of isolates was initially carried out based on morphological criteria and further confirmed by molecular studies. Molecular identification was performed by PCR assays using species-specific primers. The results showed that more than 40% (3/7) of the isolates belong to the F. culmorum species. This research also revealed, for the first time, the presence of F. cerealis (F. crookwellense) in Algeria. Finally, the use of primer assemblies allowed to highlight that the majority of isolates were of the DON chemotype; only two isolates were found to be NIV chemotype.