Breaking the PAM barrier: CRISPR-Cas12a-based ultrasensitive detection of single-nucleotide variations
Zhengdong Ai, Xiang Li, Liyuan Deng, Hao Yuan, Dapeng Zhou, Changjun Hou, Shiying Zhou
Topics & Concepts
Computer scienceScope (computer science)Computational biologyNucleic acid detectionIdentification (biology)Biochemical engineeringRisk analysis (engineering)NanotechnologyAptamerNucleic acidArtificial intelligenceProtein detectionLimitingCRISPRClinical diagnosisCRISPR and Genetic EngineeringGenomics and Rare DiseasesGenomic variations and chromosomal abnormalities