Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure
Katsuhiko Abe, Hiroshi Sugiyama, Masayuki Endo
Abstract
We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease. The activity of Cas9 incorporated inside the ring-shaped DNA origami was completely suppressed. After photoirradiation, Cas9 was released, and its activity for selective dsDNA cleavage was restored.
Topics & Concepts
CRISPRNanostructureNanotechnologyDNA origamiDNACas9Materials scienceChemistryBiochemistryGeneAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryCRISPR and Genetic Engineering