Development of <i>in vitro</i> model of exosome transport in microfluidic gut-brain axis-on-a-chip
Gwang Myeong Seo, Hongki Lee, Yeon Jae Kang, Donghyun Kim, Jong Hwan Sung
Abstract
situation of convective flow in blood vessels and convective and diffusive transport in the tissue interstitium. Here, fluorescence-labeled exosome was produced by transfection of HEK-293T cells with CD63-GFP plasmid. We observed in real time the secretion of CD63-GFP-exosomes by the transfected HEK-293T cells in the chip, and transport of the exosomes to neuronal cells and analyzed the dynamics of GFP-exosome movement. Our model is expected to enhance understanding of the roles of exosome in GBA.
Topics & Concepts
ExosomeMicrofluidicsMicrofluidic chipIn vitroChipNanotechnologyMicrovesiclesCell biologyBiologyEngineeringMaterials scienceGeneBiochemistrymicroRNAElectrical engineeringExtracellular vesicles in diseaseLattice Boltzmann Simulation StudiesMicrofluidic and Bio-sensing Technologies