Prevalence of Colistin Resistance in <i>Escherichia coli</i> in Eastern Turkey and Genomic Characterization of an <i>mcr-1</i> Positive Strain from Retail Chicken Meat
Mehmet Cemal Adıgüzel, Alper Baran, Zuowei Wu, Seyda Cengiz, Lei Dai, Cihan Öz, Esma Ozmenli, Débora Brito Goulart, Orhan Şahin
Abstract
Colistin is one of the most effective antibiotics against multidrug resistant Gram-negative bacteria. However, the recent emergence of plasmid-borne mobilized colistin resistance ( mcr ) genes is considered a serious antimicrobial resistance challenge worldwide. In this study, we report detection of an mcr-1 carrying Escherichia coli isolate (named ATAVET mcr -1 Turkey) from retail raw chicken meat in Turkey. Of the 11 (from 500 total tested) phenotypically colistin-resistant isolates, 1 was shown to carry the mcr-1 gene by PCR. Whole-genome sequencing indicated that mcr-1 was located on a ∼13 kb-long contig that was almost identical to the corresponding part in pZJ1635, an IncI2 plasmid encoding mcr-1 in the same genetic context in another E. coli strain. In addition, ATAVET mcr -1 Turkey harbored bla CTX-M-8 , qnrB19 , mdf (A), tet (A), sul2 , aph (3″)-Ib, aph (6)-Id, and floR resistance genes. Phylogenetic analysis based on whole genome and multilocus sequence typing indicated that ATAVET mcr -1 Turkey was more closely related to mcr-1 carrying E. coli isolates from food and human clinical samples previously reported from different parts of the world than to those from Turkey. These findings further emphasize the worldwide emergence and spread of mcr meditated colistin resistance in bacteria with zoonotic potential within animals and the food chain.