A Silent Operon of Photorhabdus luminescens Encodes a Prodrug Mimic of GTP
Negar Shahsavari, Boyuan Wang, Yu Imai, Miho Mori, Sangkeun Son, Libang Liang, Nils Böhringer, Sylvie Manuse, Michael F. Gates, Madeleine Morrissette, Rachel Corsetti, Josh L. Espinoza, Christopher L. Dupont, Michael T. Laub, Kim Lewis
Abstract
and then tested 200-fold concentrated material for activity. This resulted in the isolation of a novel antimicrobial, 3'-amino 3'-deoxyguanosine (ADG), active against E. coli. ADG is an analog of guanosine and is converted into an active ADG-TP in the cell. ADG-TP inhibits transcription and probably numerous other GTP-dependent targets, such as FtsZ. Natural product prodrugs have been uncommon; discovery of ADG broadens our knowledge of this type of antibiotic.
Topics & Concepts
BiologyPhotorhabdus luminescensOperonPhotorhabdusBiochemistryGuanosineGTP'Escherichia coliMutantMicrobiologyGeneEnzymeEntomopathogenic Microorganisms in Pest ControlInsect symbiosis and bacterial influencesInsect Resistance and Genetics