A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis
Ankita J. Sachla, Alexander J. Alfonso, John D. Helmann
Abstract
gRNA, genome editing can be directed to any of nearly 4,000 gene disruptants within the existing BKE collection of strains. Repair templates can be engineered as PCR products, or specific alleles and constructions can be transformed as chromosomal DNA, thereby bypassing the need for plasmid construction. The described method is rapid and facilitates a wide range of genome manipulations.
Topics & Concepts
CRISPRGuide RNACas9PlasmidGenome editingBiologyBacillus subtilisComputational biologyGenome engineeringTrans-activating crRNAGeneticsGeneGenomeBacteriaCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsBacterial Genetics and Biotechnology