Identification of 7 000–9 000 Proteins from Cell Lines and Tissues by Single-Shot Microflow LC–MS/MS
Yangyang Bian, Matthew The, Piero Giansanti, Julia Mergner, Runsheng Zheng, Mathias Wilhelm, Alexander Boychenko, Bernhard Küster
Abstract
roots, 7 500 from mouse thymus, and 7 300 from human breast cancer cells in 3 h of analysis time in a single run. The dynamic range of protein quantification measured by the iBAQ approach spanned 5 orders of magnitude and replicate analysis showed that the median coefficient of variation was below 20%. Together, this study shows that ssDDA by μLC-MS/MS is a robust method for comprehensive and large-scale proteome analysis and which may be further extended to more rapid chromatography and data independent acquisition approaches in the future.̀.
Topics & Concepts
ChemistryProteomeChromatographyMass spectrometryProteomicsOrbitrapFractionationSample preparationAnalytical Chemistry (journal)Computational biologyBiochemistryBiologyGeneAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies