Development of a novel polymerase spiral reaction (PSR) assay for rapid and visual detection of Clostridium perfringens in meat
Arockiasamy Arun Prince Milton, Kasanchi M. Momin, Sandeep Ghatak, G. Bhuvana Priya, M. Angappan, Samir Das, K. Puro, Rajkumari Sanjukta, I. Shakuntala, Arnab Sen, B.K. Kandpal
Abstract
in 38 of 74 pork samples. Therefore the specificity, sensitivity, negative predictive value, positive predictive value and accuracy rate of the developed PSR assay were 100%. The developed PSR assay is easy to perform, rapid, affordable, permitting sophisticated-equipment free amplification and naked eye interpretation. This is the initial report in which the PSR assay was optimized for the detection of
Topics & Concepts
Clostridium perfringensPolymerase chain reactionDNA extractionAssay sensitivityPredictive valueBiologyDetection limitMicrobiologyMolecular biologyChromatographyChemistryBacteriaMedicineGenePathologyBiochemistryGeneticsInternal medicineAlternative medicineClostridium difficile and Clostridium perfringens researchBacterial Identification and Susceptibility TestingListeria monocytogenes in Food Safety