Intrinsic Dual-Emitting Carbon Quantum-Dot-Based Selective Ratiometric Fluorescent Mercaptopurine Detection
Bahar Saboorizadeh, Rouholah Zare‐Dorabei
Abstract
Mercaptopurine (6-MP), an immunosuppressive drug, has been widely prescribed for treating leukemia and autoimmune diseases. The level of the 6-MP drug in body fluids is of great interest due to the severe health problems related to its overdose. This study used a facile microwave preparation route to synthesize carbon quantum dots (CQDs) using glutathione and formamide as carbon sources. The obtained monodispersed quantum dots showed dual fluorescence emission with a sensitive affinity toward the 6-MP drug. The sensor’s response was optimized by tuning the temperature, pH, and volume ratio of the probe. The prepared ratiometric fluorescence method showed accurate measurements for determining mercaptopurine in aqueous solutions in the concentration range of 1.4–7.6 mg L–1 with the limit of detection of 1.3 mg L–1. The sensor’s performance was assessed in complex solutions, human urine, and human plasma sample and recovery values in the range of 88–127% were obtained. The reliable dual fluorometric sensor showed promising results for 6-MP determination and potential application for the determination of other chemical and biochemical species.