Autophagy protects murine preputial glands against premature aging, and controls their sebum phospholipid and pheromone profile
Heidemarie Rossiter, Dragan Copic, Martin Direder, Флориан Грубер, Samuele Zoratto, Martina Marchetti‐Deschmann, Christopher Kremslehner, Michaela Sochorová, Ionela‐Mariana Nagelreiter, Veronika Mlitz, Maria Buchberger, Barbara Lengauer, Bahar Golabi, Supawadee Sukseree, Michael Mildner, Leopold Eckhart, Erwin Tschachler
Abstract
ABBREVIATIONS: ATG7: autophagy related 7; BODIPY: boron dipyrromethene; DAG: diacyl glycerides; DBI: diazepam binding inhibitor; GFP: green fluorescent protein; KRT14: keratin 14; HPLC-MS: high performance liquid chromatography-mass spectrometry; LD: lipid droplet; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MSI: mass spectrometric imaging; ORO: Oil Red O; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PG: preputial gland; PLIN2: perilipin 2; PtdIns: phosphatidylinositol; PL: phospholipids; POPC: 1-palmitoyl-2-oleoyl-PC; PS: phosphatidylserine; qRT-PCR: quantitative reverse transcribed PCR; SG: sebaceous gland; scRNAseq: single-cell RNA sequencing; TAG: triacylglycerides; TLC: thin layer chromatography.