Confounding factors from inducible systems for spatiotemporal gene expression regulation
Rob C. I. Wüst, Riekelt H. Houtkooper, Johan Auwerx
Abstract
Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, including whole-body endocrinology and disturbances in the gut microbiome and in mitochondrial and metabolic function.
Topics & Concepts
BiologyPhenotypeGeneConfoundingFunction (biology)OrganismMicrobiomeGene expressionRegulation of gene expressionGeneticsComputational biologyBioinformaticsPhysiologyInternal medicineMedicineCRISPR and Genetic EngineeringVirus-based gene therapy researchMitochondrial Function and Pathology