Production of Bioplastic (Polyhydroxybutyrate) with Local <i>Bacillus megaterium</i> Isolated from Petrochemical Wastewater.
Saba Amiri Kojuri, Khosro Issazadeh, Zoheir Heshmatipour, Mirsasan Mirpour, Saeid Zarrabi
Abstract
BACKGROUND: Polyhydroxybutyrate is a biodegradable plastic produced by some bacteria and can completely be replaced with petroleum based non-degradable plastics. OBJECTIVES: This study was done to isolate and identify one local strain with a high-production ability for industrial purposes. MATERIAL AND METHODS: H NMR. To increase more production of polyhydroxybutyrate, the effect of different factors including carbon, nitrogen, pH, and temperature were assessed. RESULTS: named saba.zh was selected as better isolation. 16S rRNA nucleotide sequence of bacterium was assigned accession number: MN519999 in the NCBI database. The optimal conditions to increase the production of polyhydroxybutyrate, are using glucose as a carbon source, ammonium sulfate as the nitrogen source, in the condition with having pH 7 and temperature 30 °C. After optimizing, the production of PHB increased from 56.51% to 85.41%. CONCLUSIONS: saba.zh, due to better polymer yield, is a potent PHB producer which can be used for PHB industrial production.