Kuragel: A biomimetic hydrogel scaffold designed to promote corneal regeneration
Parinita Agrawal, Anil Tiwari, Suvro Kanti Chowdhury, Mehak Vohra, Abha Gour, Neha Waghmare, Utkarsh Bhutani, S Kamalnath, Bharti Sangwan, Jyoti Rajput, Ritu Raj, Nisha P Rajendran, Ajith V. Kamath, Ramez I. Haddadin, Arun Chandru, Veena Sangwan, Tuhin Bhowmick
Abstract
Cornea-related injuries are the most common cause of blindness worldwide. Transplantation remains the primary approach for addressing corneal blindness, though the demand for donor corneas outmatches the supply by millions. Tissue adhesives employed to seal corneal wounds have shown inefficient healing and incomplete vision restoration. We have developed a biodegradable hydrogel - Kuragel, with the ability to promote corneal regeneration. Functionalized gelatin and hyaluronic acid form photo-crosslinkable hydrogel with transparency and compressive modulus similar to healthy human cornea. Kuragel composition was tuned to achieve sufficient adhesive strength for sutureless integration to host tissue, with minimal swelling post-administration. Studies in the New Zealand rabbit mechanical injury model affecting corneal epithelium and stroma demonstrate that Kuragel efficiently promotes re-epithelialization within 1 month of administration, while stroma and sub-basal nerve plexus regenerate within 3 months. We propose Kuragel as a regenerative treatment for patients suffering from corneal defects including thinning, by restoration of transparency and thickness.