Exploration of Remarkably Potential Multitarget-Directed N-Alkylated-2-(substituted phenyl)-1<i>H</i>-benzimidazole Derivatives as Antiproliferative, Antifungal, and Antibacterial Agents
Ngoc‐Kim‐Ngan Phan, Thi‐Kim‐Chi Huynh, Hoang-Phuc Nguyen, Quoc‐Tuan Le, Thi‐Cam‐Thu Nguyen, Kim‐Khanh‐Huy Ngo, Thi‐Hong‐An Nguyen, Khoa Anh Ton, Khac‐Minh Thai, Thi‐Kim‐Dung Hoang
Abstract
High Resolution Image Download MS PowerPoint Slide Improving lipophilicity for drugs to penetrate the lipid membrane and decreasing bacterial and fungal coinfections for patients with cancer pose challenges in the drug development process. Here, a series of new N-alkylated-2-(substituted phenyl)-1 H -benzimidazole derivatives were synthesized and characterized by 1 H and 13 C NMR, FTIR, and HRMS spectrum analyses to address these difficulties. All the compounds were evaluated for their antiproliferative, antibacterial, and antifungal activities. Results indicated that compound 2g exhibited the best antiproliferative activity against the MDA-MB-231 cell line and also displayed significant inhibition at minimal inhibitory concentration (MIC) values of 8, 4, and 4 μg mL –1 against Streptococcus faecalis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus compared with amikacin. The antifungal data of compounds 1b, 1c, 2e, and 2g revealed their moderate activities toward Candida albicans and Aspergillus niger, with MIC values of 64 μg mL –1 for both strains. Finally, the molecular docking study found that 2g interacted with crucial amino acids in the binding site of complex dihydrofolate reductase with nicotinamide adenine dinucleotide phosphate.