Mn <sup>2+</sup> -induced structural flexibility enhances the entire catalytic cycle and the cleavage of mismatches in prokaryotic argonaute proteins
Lirong Zheng, Bingxin Zhou, Yu Yang, Bing Zan, Bozitao Zhong, Banghao Wu, Yan Feng, Qian Liu, Liang Hong
Abstract
in solution, significantly promote the catalytic cycle of endonucleases and the tolerance of cleavage to mismatches. This flexibility enhancement mechanism serves as a general strategy employed by Ago proteins from diverse prokaryotes to accomplish their catalytic functions and provide useful information for Ago-based precise molecular diagnostics.
Topics & Concepts
ArgonauteNucleic acidCleavage (geology)DNAChemistryRNACatalysisCell biologyCombinatorial chemistryComputational biologyBiologyBiochemistryGeneRNA interferencePaleontologyFracture (geology)Advanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanismsDNA and Nucleic Acid Chemistry