Development of a CRISPR-SaCas9 system for projection- and function-specific gene editing in the rat brain
Haojie Sun, Su Fu, Shuang Cui, Xiang‐Sha Yin, Xiaoyan Sun, Xuetao Qi, Kun Cui, Jiaxin Wang, Longyu Ma, Fengyu Liu, Feifei Liao, Xinhong Wang, Ming Yi, You Wan
Abstract
(CREB-binding protein), a sample target gene, in specific neuronal subpopulations in the medial prefrontal cortex, and demonstrated the significance of the projection- and function-specific CRISPR-SaCas9 system in revealing neuronal and circuit basis of memory. The high efficiency and specificity of our projection- and function-specific CRISPR-SaCas9 system could be widely applied in neural circuitry studies.
Topics & Concepts
CRISPRGenome editingFunction (biology)GeneProjection (relational algebra)Brain functionBiologyComputational biologyComputer scienceNeuroscienceGeneticsAlgorithmCRISPR and Genetic EngineeringRetinal Development and DisordersNeuroscience and Neural Engineering