Perfecting Targeting in CRISPR
Hainan Zhang, Tong Li, Yidi Sun, Hui Yang
Abstract
CRISPR-based genome editing holds promise for genome engineering and other applications in diverse organisms. Defining and improving the genome-wide and transcriptome-wide specificities of these editing tools are essential for realizing their full potential in basic research and biomedical therapeutics. This review provides an overview of CRISPR-based DNA- and RNA-editing technologies, methods to quantify their specificities, and key solutions to reduce off-target effects for research and improve therapeutic applications.
Topics & Concepts
CRISPRGenome editingBiologyComputational biologyGenomeGenome engineeringTranscriptomeGeneticsGeneGene expressionCRISPR and Genetic EngineeringRNA regulation and diseaseVirus-based gene therapy research