Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System
Donghyun Park, Samuel Steiner, Meng Shao, Craig R. Roy, Jun Liu
Abstract
. Importantly, assembled Dot/Icm machines were not present in the infectious SCV. The appearance of the assembled Dot/Icm machine correlated with the transition of the SCV to the LCV intracellularly. Furthermore, temporal characterization of C. burnetii morphological changes revealed regions of the inner membrane that invaginate to form tightly packed stacks during the LCV-to-SCV transition at late stages of infection, which may enable the SCV-to-LCV transition that occurs upon infection of a new host cell. Overall, these data establish how C. burnetii developmental transitions control critical bacterial processes to promote intracellular replication and transmission.
Topics & Concepts
Coxiella burnetiiBiologySecretionVirologyBiochemistryLegionella and Acanthamoeba researchVector-borne infectious diseasesInvertebrate Immune Response Mechanisms