Tracking Mutual Interactions of Mitochondria/Lysosomes/Lipid Droplets in DILI and NAFLD with a Viscosity and Peroxynitrite-Sensitive Single Fluorescent Probe
Chunhua Fan, Xionghao Xu, Bo Zhao, Tao Jiang, Tianxin Liu, Chaewoon Cho, Juyoung Yoon, Zhengliang Lü
Abstract
and pH of lysosomes. Using a meticulously designed molecular logic gate, it is possible to achieve real-time tracking of three organelles in a synchronized manner, revealing a significant increase in their fusion frequency, mutual contacts, and interactions. This comprehensive study underscores the potential of developing innovative multifunctional, multitargeted probes, which could significantly improve early diagnosis and treatment options for related diseases.
Topics & Concepts
ChemistryPeroxynitriteFluorescenceBiophysicsLipid dropletIntracellularOrganelleNonalcoholic fatty liver diseaseMitochondrionReactive oxygen speciesStimulationBiochemistryViscosityPolarity (international relations)Fluorescence microscopeTracking (education)mitochondrial fusionNanotechnologyPeroxynitrous acidLaser-induced fluorescenceSubcellular localizationCell biologyCytoplasmFatty liverLipid oxidationLipid peroxidationLipid metabolism and biosynthesisAdipose Tissue and MetabolismMitochondrial Function and Pathology